ABSTRACT
OBJECTIVE: To establish a rapid ultra-high performance liquid chromatography method for determining teicoplanin concentration in human plasma. METHODS: The samples were chromatographed on Waters Acquity UPLC BEH-C18 column using gradient elution method(mobile phase A:20 mmol•L-1 ammonium acetate;mobile phase B:acetonitrile). The absorption wavelength was set at 215 nm. The column temperature were maintained at 35 ℃. The teicoplanin concentration was calculated by internal standard method and external standard method, respectively. The results of the two methods were compared by paired t-test using SPSS statisticals software. RESULTS: The liner range of the calibration curve for teicoplanin was 3.15-100 μg•mL-1(r=0.999 9). The concentrations calculated by the two methods had good correlation without significant difference. CONCLUSION: This method is proved to be rapid, sensitive and suitable for the therapeutic drug monitoring and pharmacokinetic investigation of teicoplanin in human plasma.